Methods

We use and provide training for classical virological assays as well as digital PCR analysis, nanopore sequencing, infectious (BSL-3) live cell imaging, and high throughput screening assays.

A549-AT infection model for monitoring SARS-CoV-2 replication

Using the Sleeping Beauty transposon system (Kowarz et al. 2015), we established a reliable and adaptable cellular infection model based on A549 lung cells. This model facilitated our high-throughput SARS-CoV-2 infection studies and enabled live cell imaging at a significantly reduced cost.

Read the paper to find out more: Widera et al. 2021 Front Microbiol. 2021 Jul 29:12:701198. doi: 10.3389/fmicb.2021.701198. eCollection 2021.

Read our interview on Generating a cellular infection model for monitoring SARS-CoV-2 replication in the Tecan Journal:
https://www.tecan.com/tecan-journal/generating-a-cellular-infection-model-for-monitoring-sars-cov-2-replication

Are you also intersted in work with the A549-AT cells?

• Infectious cell culture and replication studies (HIV-1 & SARS-CoV-2)
• Infectious (live) cell Imaging
• Gene expression and absolute quantification with (d)PCR and ONT
• High throughput infection models
• Lentiviral reporter assays
• Isolation of (viral) nucleic acids from cell culture and wastewater
• Generation of reporter cell lines