Our research aims to detect and characterize the genotypes of human pathogenic RNA viruses in both infected cells and wastewater samples. We are particularly interested in deciphering the genotypes of HIV-1 and SARS-CoV-2 variants, with an emphasis on understanding viral gene expression and immune escape.
The human immunodeficiency virus type 1 (HIV-1) and the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) are significant human pathogenic RNA viruses. They have recently crossed from zoonotic diseases in animals to infect humans. Both viruses have a rather small but complex genome and are characterized by high mutation frequencies and rapid evolutionary adaptation to new conditions. They rely on cellular proteins and processes, including interactions with RNA-binding proteins, to efficiently utilize their relatively small viral genomes.
- In our research group, we focus on the detection, viral genetics, and viral-host cell interactions of two clinically significant pathogens. Specifically, we aim to:
- Utilize digital PCR and nanopore sequencing for molecular wastewater epidemiology, and phenotypically characterize genotypically relevant viral variants.
- Develop and optimize molecular and cellular detection and analysis methods for high-throughput analysis.
- Enhance our understanding of viral RNA processing, alternative splicing, transcription, and programmed ribosomal frameshifting, evaluating these processes as potential targets for novel antiviral therapies.
AIDS/HIV-1
DFG Priority Program – SPP 1923: “Innate Sensing and Restriction of Retroviruses”. Project: Interferon-induced alteration of cellular factors affecting HIV alternative splicing, nuclear mRNA export and LTR transcription. More info: https://www.spp1923.de/
COVID-19/SARS-CoV-2
LOEWE Priority Program CoroPan: Human and zoonotic coronaviruses: conserved targets for new therapeutic options in future pandemics. Project The RNA milieu in coronavirus-infected cells, funded by HMWK, more info here.
Innovative Therapeutics (TheraNova), Rhine/Main Region, funded by the Fraunhofer Institute of Translational Medicine and Pharmacology ITMP and
HMWK, more info here.
Wastewater based molecular epidemiology
Collaborative project WBEready: Wastewater-based Epidemiology and Preparedness: Research Needs for a Roadmap to Develop Adaptive Monitoring Capacities in Public Health Services; funded by BMG.
More info: https://www.wbeready.de